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in situ hybridization
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对于极微量的靶序列的扩增可以采用下列哪种PCR技术？( )A、AP-PCR方法(arbitrarily primed PCR)B、通用引物-PCR方法(general primer-mediated PCR，GP-PCR)C、套式PCR(nested primers-polymerase chainreaction，NP-PCR)D、多重PCR(multiplex PCR)E、原位PCR技术(in situ PCR，ISPCR)
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第二组The only attention that heat exchanger should require is to ensure that the heat transfer surfaces remain substantially clean and the flow passages generally clear of obstruction. Indication that undue fouling is occurring is given by a progressive increase in the temperature difference between the two fluids, over a period of time, usually accompanied by a noticeable rise in pressure loss at a given flow.Fouling on the sea-water side is the most usual cause of deterioration in performance. The method of cleaning the sea water-side surfaces depends on the type of heat exchanger. With shell-and-tube heat exchangers, the removal of the header covers or, m the case of the smaller heat exchangers, the headers themselves, will provide access to the tubes. Obstructions, dirt, scale etc., can then be removed, using the tools provided by the heat exchanger manufacturer. Flushing through with fresh water is recommended before a heat exchanger is returned to service. In some applications, such as piston oil cooling, progressive fouling may take place on the outside of the tubes. Most manufactures recommend a chemical flushing pr6cess to remove this in situ~ without dismantling the heat exchanger.Plate heat exchangers may be cleaned by unclamping the stack of plates and mechanically cleaning the surface of each plate as recommended by the manufacturers. The plate seals may require replacement from time to time and here the manufacturers instructions should be closely followedBy which of the following indication, can we conclude that the lube oil-sea water heat exchanger is unduly fouled?A.the heat exchanger lube oil inlet temperature loweringB.the heat exchanger sea-water outlet pressure riseC.the heat exchanger temperature difference between lube oil and sea water increasingD.the heat exchanger pressure difference between sea water inlet and outlet decreasing
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单选题与蛋白质芯片技术原理相似的技术是（）。A
Northern blottingB
Western blottingC
Southern blottingD
Eastern blottingE
dot blot hybridization
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单选题Le pays _____ je viens est situé à l’autre bout du monde, c’est pourquoi tout me semble nouveau et intéressant ici.A
oùB
d’oùC
dontD
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问答题杂交育种（breeding by hybridization）
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